Modifying Enzymes
New England Biolabs, Inc. T4 PDG (T4 Endonuclease V) – 2000 units
T4 PDG (pyrimidine dimer glycosylase) has both DNA glycosylase and AP lyase activity. The 16 kd protein recognizes cis-syn-cyclobutane pyrimidine dimers caused by UV irradiation. The enzyme cleaves the glycosyl bond of the 5' end of the pyrimidine dimer and the endonucleolytic activity cleaves the phosphodiester bond at the AP site.
New England Biolabs, Inc. Bacteroides Heparinase III – 35 units
Bacteroides Heparinase III, also called Heparin Lyase III, is active on both heparin and heparan sulfate. Bacteroides Heparinase III can cleave the glycosidic bond between hexosamines and either iduronic acid or glucuronic acid residues. It is active in the presence of 6-sulfation. The reaction yields oligosaccharide products containing unsaturated uronic acids, which can be detected by UV spectroscopy at 232 nm
American Research Products Inc LIPASE KIT COLORIMETRIC BA0052
5000265510 LIPASE KIT COLORIMETRIC BA0052
New England Biolabs, Inc. Bacteroides Heparinase II – 80 units
Bacteroides Heparinase II (also called Heparin Lyase II) is cloned from Bacteroides eggerthii. It is a low specificity enzyme that is active on both heparin and heparan sulfate. Bacteroides Heparinase II cleaves the glycosidic bond between N-sulfated and glucuronic or iduronic acid residues. The reaction yields oligosaccharide products containing unsaturated uronic acids which can be detected by UV spectroscopy at 232 nm. When used alone this enzyme rarely yields complete depolymerization of a polysaccharide chain, however disaccharide analysis is enhanced when used in combination with Heparinase I and III.
New England Biolabs, Inc. Thermostable 5' App DNA/RNA Ligase – 10 reactions
Thermostable 5' App DNA/RNA Ligase is a point mutant of catalytic lysine of RNA ligase from Methanobacterium thermoautotrophicum. This enzyme is ATP independent. It requires a 5' pre-adenylated linker for ligation to the 3' -OH end of either RNA or single stranded DNA (ssDNA). The enzyme is also active in ligation of RNA with 2' -O-methylated 3 end to 5-adenylated linkers. The optimal temperature for ligation reaction is 60-65°C. The mutant ligase is unable to adenylate the 5' -phosphate of RNA or ssDNA, which reduces the formation of undesired ligation products (concatemers and circles). The ability of the ligase to function at 65°C might reduce the constraints of RNA secondary structure in RNA ligation experiments.
New England Biolabs, Inc. DNase I (RNase-free) – 5000 units
DNase I, (RNase-free) is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5' -phosphorylated and 3' -hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids.
Sigma Aldrich Fine Chemicals Biosciences Neuraminidase (Sialidase) from Clostridium perfringens |
Neuraminidase (Sialidase) from Clostridium perfringens |
Sigma Aldrich Fine Chemicals Biosciences Topoisomerase II alpha hum
Topoisomerase II is encoded by TOP2A gene which is located on 17q21.2 in the human chromosome. It is essential for controlling the topological state of DNA by reversibly breaking the DNA strands during replication recombination and transcription. TOP2A expression increases at late S phase and during G2/M of the cell cycle and decreases after mitosis. Polymorphism with TOP2A causes chromosome instability and tumorigenesis. TOP2A causes tumor proliferation and invasiveness in various cancer types like breast cancer ovarian cancer and prostate cancer.
American Research Products Inc GENIEPURE WATER DNASE RNASE FR
5000265171 GENIEPURE WATER DNASE RNASE FR
New England Biolabs, Inc. Bst DNA Polymerase, Large Fragment – 8000 units
Bst DNA Polymerase, Large Fragment is the portion of the Bacillus stearothermophilus DNA Polymerase protein that contains the 5' to 3' polymerase activity, but lacks 5' to 3' exonuclease activity.
New England Biolabs, Inc. α-N-Acetylgalactosaminidase - 3000 units
a-N-Acetylgalactosaminidase is an exoglycosidase that catalyzes the hydrolysis of terminal, non-reducing a-N-Acetylgalactosamine residues from oligosaccharides and N-glycans attached to proteins.
New England Biolabs, Inc. Exonuclease I Reaction Buffer – 6 ml
This is the NEB reaction buffer supplied with Exonuclease I. New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme.
New England Biolabs, Inc. Exonuclease III (E.coli) – 5000 units
Catalyzes the stepwise removal of mononucleotides from 3'-hydroxyl termini of duplex DNA. A limited number of nucleotides are removed during each binding event, resulting in coordinated progressive deletions within the population of DNA molecules. The preferred substrates are blunt or recessed 3'-termini, although the enzyme also acts at nicks in duplex DNA to produce single-strand gaps. 3'-protruding termini are resistant to cleavage; the degree of resistance depends on the length of the extension,with extensions 4 bases or longer being essentially resistant to cleavage. Exonuclease III activity depends partially on helical structure and displays sequence dependence (C>A=T>G). Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specific applications. Exonuclease III has also been reported to have RNase H, 3'-phosphatase and AP-endonuclease activities.